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Biochemie II

AG Prof. Dr. Monique Aumailley

  • Wechselwirkungen zwischen Zellen und der extrazellulären Matrix

o   Modulation der zellulären Aktivitäten durch Laminin-Isoformen

o   Laminin-bindende Integrine und andere Rezeptoren kontrollierte Migration von Epithelzellen

o   Die Rolle von Lamininen in der Bildung und Umbau von Epithelbasalmembran

o   Die Rolle von Rho GTPasen in "Laminin signaling"

aumailleySpamProtectionuni-koeln.de

AG Prof. Dr. Franz-Georg Hanisch

  • Molecular Glycobiology

Our research efforts focus on mucin-type O-glycosylation, initially concentrating on tumor-associated carbohydrate antigens, later in a more general biological context. As model protein we investigated the structural aspects of MUC1 glycosylation, the first human mucin described and one of the most promising tumor markers with impact in diagnosis and in immunotherapy. Beyond a mere profiling of glycans expressed on the mucin from the normal (lactating) breast or from breast cancer cells, we tried to determine the changes in density and the sites of O-glycosylation associated with cancer development. In later studies we looked for the influence of the sites glycosylated on MUC1 tandem repeat peptides on their immunological properties (processing in the class I and class II pathways) to prepare the basis for a vaccine development. Other projects focus on the investigation of additional rare O-Glycosylation-Types and their control by peptidic alements as well as on development of new techniques with regard to glycomics in SDS-gel-based platforms and to glycoproteomic approaches aiming at the improvement of O-glycoprotein identification.

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AG Dr. Ursula Hartmann

  • Charakterisierung extrazellulärer, Calcium-bindender Proteine, Molekulare Neurobiologie

We are interested in a certain family of matrix proteins, the so called EC domain proteins. The members of these family are multidomain proteins characterised by a domain which contains two calcium-binding EF-hand motifs and was therefore termed extracellular calcium-binding (EC) domain. The prototype of these family is BM-40 (also called osteonectin or SPARC), a glycoprotein that has been implicated in cell growth and tissue remodeling. It. was the first extracellular protein that was shown to contain EF-hand motifs, demonstrating that this motif is not confined to intracellular proteins. EC domains are also found in the proteins SC1/hevin, tsc36 and testican/Spock where they are always preceded by a FS domain. During the last years we could identify four novel members of the EC domain family. Two share the domain organisation of testican/Spock and hence were termed testican-2 and testican-3. The two other proteins show a unique domain organisation and were termed SMOC-1 and SMOC-2, where SMOC stands for Secreted Modular Calcium-binding protein.

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AG PD Dr. Catherin Niemann

  • Molecular function of Lef1 mutations (E45K/S61P and DNLef1) in epithelial tissues and cancer, SFB 829, CMMC

The overall goal is to understand cellular and molecular mechanisms that control adult epithelial stem cells, both under homeostatic and pathological conditions. Our research is focussing on epithelial stem cells of the skin that generate a diverse set of differentiated cell lineages, including keratinocytes of the interfollicular epidermis, hair follicles and sebaceous glands. We are investigating regulatory pathways of stem cell activation and lineage selection to explore their therapeutic value and we aim to identify the underlying molecular mechanisms of stem and progenitor cell activation in the process of epidermal tumour formation.

cnieman1@uni-koeln.de

AG PD Dr. Markus Plomann

  • Zelluläre Neurobiologie, Intrazellulärer Vesikeltransport

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AG PD Dr. Gerhard Sengle

  • Interactions of growth factors with the extracellular matrix

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AG Prof. Dr. Raimund Wagener

  • Struktur und Funktion von VWA-Domänen enthaltenden Proteinen der extrazellulären Matrix

raimund.wagenerSpamProtectionuni-koeln.de